5. Replication of plasmids with a pR6K origin (oriR6K) requires a protein called pi (encoded by the pir gene). What would happen if a plasmid with oriR6K that encodes ampicillin resistance (AmpR) and carries the putA+ gene was introduced into a recipient that is pir+ putA(Am)? [Would the plasmid replicate? Would the plasmid integrate into the chromosome? Would the cell be Put+ or Put- and why?]
ANSWER: See figure below.
What would happen if a plasmid with oriR6K that encodes ampicillin resistance (Ampr) and carries the putA+ gene was introduced into a recipient that is pir putA(Am)? [Would the plasmid replicate? Would the plasmid integrate into the chromosome? Would the cell be Put+ or Put- and why?]
ANSWER: See figure below.
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